Virtual PCR Simulator

Step 0: Make Master Mix

Select Enzyme: Taq Polymerase (Optimal Temp: 72°C) Pfu Polymerase (Optimal Temp: 75°C) Q5 Polymerase (Optimal Temp: 70°C)

Add Buffer Add dNTP

Next

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Step 1: Add Template and Primers

DNA Sequence (500 bp): Generate DNA Sequence Enter Desired PCR Product Length Range: Suggest Primers Enter Forward Primer: Enter Reverse Primer:

Back Next

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Step 2: PCR Machine Settings

Denaturation Temperature (°C): Annealing Temperature (°C): Extension Temperature (°C): Number of Cycles: Back Run PCR

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Step 3: Electrophoresis

Markers are shown every 100 bp.

Run Electrophoresis Stop Electrophoresis Back Show Summary Next

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Step 4: Gel Extraction and Sequencing

Extract PCR Product from Gel Sequence PCR Product Back

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