Junpei Takano

Research Interest

Our lab studies how plants sense nutrients and maintain their homeostasis by controlling abundance and intracellular localization of membrane transport proteins. Our specific interests include mechanisms underlying the boron sensing by a borate transporter for its own degradation and the polar localization of transport proteins in the plasma membrane. We are also interested in development of nutrient efficient crops utilizing the molecular mechanisms of nutrient transport.

Keywords:
Plant nutrition, membrane transport, membrane traffic, nutrient sensing, boron, phosphate use efficiency

C.V.

1995-1999 BSc. Faculty of Agriculture, The University of Tokyo
1999-2004 Ph.D. Graduate School of Agricultural and Life Sciences, The University of Tokyo (Supervisor: Dr. Toru Fujiwara) 2002-2003 Guest student, Hohenheim University, Institute of Plant Nutrition, Germany (Prof. Nico von Wirén lab.)
2004-2006 Research Fellow of JSPS, Center for Biotechnology Research, The University of Tokyo (Dr. Toru Fujiwara lab.)
2007-2008 Fellow of Human Frontier Science Program, University of Wisconsin-Madison, US, (Prof David Eide lab.)
2008-2016 Assistant professor, Graduate School of Agriculture, Hokkaido University (Prof. Satoshi Naito lab.)
2016- Professor, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
2022- Professor, Graduate School of Agriculture, Osaka Metropolitan University

Awards

Sep 2024 Prize from the Japanese Society of Soil Science and Plant Nutrition
Feb 2016 Hokkaido University President's Award for Research
Mar 2012 Hokkaido University President's Award for Research
Apr 2009 The Young Scientists’ Prize: The Commendation for Science and Technology by the Minister of Education,Culture, Sports, Science and Technology
Mar 2007 Human Frontier Science Program Award: Long term fellowship
Nov 2006 Young Researcher Prize from the foundation of agricultural sciences of Japan
Sep 2006 Young Researcher Prize from the Japanese Society of Soil Science and Plant Nutrition
Mar 2004 The University of Tokyo President's Award for Students

Editorial board member

Jan. 2023- Plant Physiology
2010-2023 Frontiers in Plant Science
Oct 2020-Sep.2022 Soil Science and Plant Nutrition

Selected Publication

Zhe Zhang, Shunsuke Nakamura, Arisa Yamasaki, Masataka Uehara, Shunsuke Takemura, Kohei Tsuchida, Takehiro Kamiya, Shuji Shigenobu, Katsushi Yamaguchi, Toru Fujiwara, Sumie Ishiguro, Junpei Takano. 2024. Arabidopsis KNS3 and its two homologs mediate endoplasmic reticulum-to-plasma membrane traffic of boric acid channels. Journal of Experimental Botany: erae380. Standard link

Akira Yoshinari, Yutaro Shimizu, Takuya Hosokawa, Akihiko Nakano, Tomohiro Uemura, Junpei Takano. 2024. Rapid Vacuolar Sorting of the Borate Transporter BOR1 Requires the Adaptor Protein Complex AP-4 in Arabidopsis. Plant Cell Physiology. doi: 10.1093/pcp/pcae096.

Keita Muro, Shoji Segami, Miki Kawachi, Nodoka Horikawa, Ayane Namiki, Komachi Hashiguchi, Masayoshi Maeshima, Junpei Takano. 2024. Localization of the MTP4 transporter to trans-Golgi network in pollen tubes of Arabidopsis thaliana. Journal of Plant Research. SharedIt link

Muthuvel JOTHI, Junpei TAKANO and Lingaraj SAHOO. 2024. Higher Boron Accumulation is Associated with Low-Boron Tolerance in Mustard. Japan Agricultural Research Quarterly. vol. 58 no.4. Share link

Muro K, Kamiyo J, Wang S, Geldner N and Takano J. 2023. Casparian strips prevent apoplastic diffusion of boric acid into root steles for excess B tolerance. Front. Plant Sci. 14:988419. doi: 10.3389/fpls.2023.988419.

Takano J, & Tanaka M. 2023. Boron-sensing mechanisms involved in boron transport regulation in plants. Journal of Plant Nutrition and Soil Science, 00, 1-8. Share link

Muthuvel Jothi, Junpei Takano. Marschner Review: Understanding the regulatory mechanisms of B transport to develop crop plants with B efficiency and excess B tolerance. Plant and Soil. DOI: 10.1007/s11104-023-05975-y.

Yoshinari A*, Hosokawa T*, Beier MP, Oshima K, Ogino Y, Hori C, Takasuka TE, Fukao Y, Fujiwara T, Takano J. 2021. Transport-coupled ubiquitination of the borate transporter BOR1 for its boron-dependent degradation. The Plant Cell. *These authors contributed equally.

Yoshinari A, Toda Y, Takano J (2021) GNOM‐dependent endocytosis maintains polar localization of the borate exporter BOR1 in Arabidopsis. Biology of the Cell.

Yoshinari A, Hosokawa T, Amano T, Beier MP, Kunieda T, Shimada T, Hara-Nishimura I, Naito S, Takano J (2019) Polar localization of the borate exporter BOR1 requires AP2-dependent endocytosis. Plant Physiology 179: 1569-1580

Fukuda M*, Wakuta S*, Kamiyo J, Fujiwara T, Takano J (2018) Establishment of genetically encoded biosensors for cytosolic boric acid in plant cell. The Plant Journal 95: 763-774 *These authors contributed equally.

Wang S, Yoshinari A, Shimada T, Hara-Nishimura I, Mitani-Ueno N, Ma JF, Naito S, Takano J (2017) Polar localization of the NIP5;1 boric acid channel is maintained by endocytosis and failitates boron transport in Arabidopsis roots. The Plant Cell 29: 824-842. 

Yoshinari A, Takano J (2017) Insights into the mechanisms underlying boron homeostasis in plants. Frontiers in Plant Science.

Takano J, Yoshinari A, Luu D (2017) Plant Aquaporin Trafficking. in Plant Aquaporins, Signaling and Communication in plants.

Yoshinari A, Fujimoto M, Ueda T, Inada N, Naito S, Takano J (2016) DRP1-dependent endocytosis is essential for polar localization and boron-induced degradation of the borate transporter BOR1 in Arabidopsis thaliana. Plant Cell Physiology 57: 1985-2000.

Takano J, Tanaka M, Toyoda A, Miwa K, Kasai K, Fuji K, Onouchi H, Naito S, Fujiwara T (2010) Polar localization and degradation of Arabidopsis boron transporters through distinct trafficking pathways. Proc Natl Acad Sci U S A. 107(11): 5220-5225.

Takano J*, Wada M*, Ludewig U, Schaaf G, von Wirén N, Fujiwara T (2006) The Arabidopsis Major Intrinsic Protein NIP5 ; 1 Is Essential for Efficient Boron Uptake and Plant Development under Boron Limitation. Plant Cell. 2006;18(June):1498-1509. *These authors contributed equally.

Takano J, Miwa K, Yuan L, von Wir én N, Fujiwara T (2005) Endocytosis and degradation of BOR1, a boron transporter of Arabidopsis thaliana, regulated by boron availability. Proc Natl Acad Sci USA 102: 12276-12281

Takano J, Noguchi K, Yasumori M, Kobayashi M, Gajdos Z, Miwa K, Hayashi H, Yoneyama T, Fujiwara T (2002) Arabidopsis boron transporter for xylem loading. Nature 420: 337-340

ResearchGate (Publication, C.V. etc.)HUSCAP (Publication from Hokudai)