研究業績

2024

Jyunna Yoshida, Yuki Kato, Ai Isogawa, Yoshikazu Tanaka, Izumi Kumagai, Ryutaro Asano, Takeshi Nakanishi, and Koki Makabe, Construction of bispecific antibodies by specific pairing between the heavy chain and the light chain using removable SpyCatcher/SnoopCatcher units, J. Biol. Eng. 18, 57 (2024).

2023

Kouki Kimura, Atsushi Kuwahara, Saori Suzuki, Takeshi Nakanishi, Izumi Kumagai, and Ryutaro Asano, Cancer therapeutic trispecific antibodies recruiting both T and natural killer cells to cancer cells, Oncol. Rep. 50, 212 (2023).
Risa Yamada, Ishin Nakahara, Izumi Kumagai, Ryutaro Asano, Takeshi Nakanishi, and Koki Makabe, Construction of IgG–Fab² bispecific antibody via intein-mediated protein trans-splicing reaction, Sci. Rep. 13, 15961 (2023).
Honoka Yamamoto, Takeshi Nakanishi, Kan-ichiro Ihara, Taro Tachibana, and Chikako Yokoyama, Generation of rat monoclonal antibodies against human epidermal growth factor receptor 2, Monoclon. Antib. Immunodiagn. Immunother. 42, 59-64 (2023).

2020

Kiattisak Lugsanangarm, Haruhiko Tamaoki, Yasuzo Nishina, Masaya Kitamura, Nadtanet Nunthaboot, Fumio Tanaka, Seiji Taniguchi, and Haik Chosrowjan, Simultaneous analyses of the rates of photoinduced charge separation and recombination between the excited flavin and tryptophans in some flavoproteins: Molecular dynamics simulation, AIP Advances 10, 105224 (2020).
Liu Xu, Kan-ichiro Ihara, Saori Yoshimura, Daijiro Konno, Akira Tachibana, Takeshi Nakanishi, and Taro Tachibana, Generation of the rat monoclonal antibody against the extracellular domain of human CD63 by DNA immunization, Monoclon. Antib. Immunodiagn. Immunother. 39, 74-76 (2020).
Ryutaro Asano, Katsuhiro Hosokawa, Shintaro Taki, Shota Konno, Ippei Shimomura, Hiromi Ogata, Mai Okada, Kyoko Arai, Masayoshi Onitsuka, Takeshi Omasa, Takeshi Nakanishi, Mitsuo Umetsu, and Izumi Kumagai, Build-up functionalization of anti-EGFR × anti-CD3 bispecific diabodies by integrating high-affinity mutants and functional molecular formats, Sci. Rep. 10, 4913 (2020).
Saki Hemmi, Ryutaro Asano, Kouki Kimura, Mitsuo Umetsu, Takeshi Nakanishi, Izumi Kumagai, and Koki Makabe, Construction of a circularly connected VHH bispecific antibody (cyclobody) for the desirable positioning of antigen-binding sites, Biochem. Biophys. Res. Commun. 523, 72-77 (2020).
Asami Ueda, Mitsuo Umetsu, Takeshi Nakanishi, Kentaro Hashikami, Hikaru Nakazawa, Shuhei Hattori, Ryutaro Asano, and Izumi Kumagai, Chemically crosslinked bispecific antibodies for cancer therapy: Breaking from the structural restrictions of the genetic fusion approach, Int. J. Mol. Sci. 21, E711 (2020).

2018

Hideaki Sanada, Kazuki Kobayashi, Takamitsu Maru, Takeshi Nakanishi, Mitsuo Umetsu, Ryutaro Asano, and Izumi Kumagai, Affinity maturation of humanized anti-epidermal growth factor receptor antibody using a modified phage-based open sandwich selection method, Sci. Rep. 8, 5414 (2018).
Tomohiro Osaki, Takeshi Nakanishi, Motoshi Aoki, Takahiro Omizu, Daisuke Nishiura, and Masaya Kitamura, Soluble expression in Escherichia coli of a single-domain antibody-tumor necrosis factor α fusion protein specific for epidermal growth factor receptor, Monoclon. Antib. Immunodiagn. Immunother. 37, 20-25 (2018).

2017

Nadtanet Nunthaboot, Kiattisak Lugsanangarm, Arthit Nueangaudom, Somsak Pianwanit, Sirirat Kokpol, Fumio Tanaka, Seiji Taniguchi, Haik Chosrowjan, Takeshi Nakanishi, and Masaya Kitamura, Photoinduced electron transfer from aromatic amino acids to the excited isoalloxazine in single mutated flavin mononucleotide binding proteins: Effect of the dimer formation on the rate and the electrostatic energy inside the proteins, Comput. Theor. Chem. 1108, 1-9 (2017).

2016

Yoshihiro Haruyama, Katsuyuki Kasai, Toshiki Yamada, Shukichi Tanaka, Yukihiro Tominari, Takahiro Kaji, Masaya Kitamura, and Akira Otomo, Development of UV light irradiation patterning of bacteriorhodopsin thin films for biomimetic functional devices, J. Nanosci. Nanotechnol. 16, 3420-3425 (2016).
Nadtanet Nunthaboot, Kiattisak Lugsanangarm, Somsak Pianwanit, Sirirat Kokpol, Fumio Tanaka, Takeshi Nakanishi, and Masaya Kitamura, Conformational difference between two subunits in flavin mononucleotide binding protein dimers from Desulfovibrio vulgaris (MF): Molecular dynamics simulation, Comput. Biol. Chem. 64, 113-125 (2016).
Nadtanet Nunthaboot, Kiattisak Lugsanangarm, Arthit Nueangaudom, Somsak Pianwanit, Sirirat Kokpol, Fumio Tanaka, Seiji Taniguchi, Haik Chosrowjan, Takeshi Nakanishi, and Masaya Kitamura, Photoinduced electron transfer from aromatic amino acids to the excited isoalloxazine in flavin mononucleotide binding protein. Is the rate in the inverted region of donor-acceptor distance not real?, J. Photochem. Photobiol. A: Chem. 326, 60-68 (2016).

2015

Tomohiro Osaki, Cai-Xia Wang, Taro Tachibana, Masayuki Azuma, Masaya Kitamura, and Takeshi Nakanishi, Generation and characterization of rat monoclonal antibodies against epidermal growth factor receptor, Monoclon. Antib. Immunodiagn. Immunother. 34, 418-422 (2015).
Tomohiro Osaki, Shingo Fujisawa, Masahiro Kitaguchi, Masaya Kitamura, and Takeshi Nakanishi, Development of a bispecific antibody tetramerized through hetero-associating peptides, FEBS J. 282, 4389-4401 (2015).

2014

Nadtanet Nunthaboot, Kiattisak Lugsanangarm, Somsak Pianwanit, Sirirat Kokpol, Fumio Tanaka, Seiji Taniguchi, Haik Chosrowjan, Takeshi Nakanishi, and Masaya Kitamura, Bell-shape dependence of the rate of ultrafast photoinduced electron transfer from aromatic amino acids to the excited flavin on the donor-acceptor distance in FMN binding proteins, Comput. Theor. Chem. 1030, 9-16 (2014).

2013

Kiattisak Lugsanangarm, Somsak Pianwanit, Arthit Nueangaudom, Sirirat Kokpol, Fumio Tanaka, Nadtanet Nunthaboot, Kumiko Ogino, Rikako Takagi, Takeshi Nakanishi, Masaya Kitamura, Seiji Taniguchi, and Haik Chosrowjan, Mechanism of photoinduced electron transfer from tyrosine to the excited flavin in the flavodoxin from Helicobacter pylori. A comparative study with flavodoxins and flavin mononucleotide binding proteins from Desulfovibrio vulgaris (Miyazaki F), J. Photochem. Photobiol. A: Chem. 268, 58-66 (2013).
Ryutaro Asano, Takashi Kumagai, Keisuke Nagai, Shintaro Taki, Ippei Shimomura, Kyoko Arai, Hiromi Ogata, Mai Okada, Fumitaka Hayasaka, Hideaki Sanada, Takeshi Nakanishi, Teemu Karvonen, Hiroki Hayashi, Yu Katayose, Michiaki Unno, Toshio Kudo, Mitsuo Umetsu, and Izumi Kumagai, Domain order of a bispecific diabody dramatically enhances its antitumor activity beyond structural format conversion: The case of the hEx3 diabody, Protein Eng. Des. Sel. 26, 359-367 (2013).
Seiji Taniguchi, Haik Chosrowjan, Fumio Tanaka, Takeshi Nakanishi, Shuta Sato, Yoshihiro Haruyama, and Masaya Kitamura, A key factor for ultrafast rates of photoinduced electron transfer among five flavin mononucleotide binding proteins: Effect of negative, positive and neutral charges at residue 13 on the rate, Bull. Chem. Soc. Jpn. 86, 339-350 (2013).
Takeshi Nakanishi, Takamitsu Maru, Kazuhiro Tahara, Hideaki Sanada, Mitsuo Umetsu, Ryutaro Asano, and Izumi Kumagai, Development of an affinity-matured humanized anti-epidermal growth factor receptor antibody for cancer immunotherapy, Protein Eng. Des. Sel. 26, 113-122 (2013).

2012

中西猛，真田英明，熊谷泉，ファージ提示系による親和性の向上，次世代医薬開発に向けた抗体工学の最前線（シーエムシー出版），47-52 (2012)．
Kiyohito Kihira, Yoshihiro Shimizu, Yasuhito Shomura, Naoki Shibata, Masaya Kitamura, Atsushi Nakagawa, Takuya Ueda, Kozo Ochi, and Yoshiki Higuchi, Crystal structure analysis of the translation factor RF3 (release factor 3), FEBS Lett. 586, 3705-3709 (2012).
Takamitsu Hattori, Mitsuo Umetsu, Takeshi Nakanishi, Satoko Sawai, Shinsuke Kikuchi, Ryutaro Asano, and Izumi Kumagai, A high-affinity gold-binding camel antibody: antibody engineering for one-pot functionalization of gold nanoparticles as biointerface molecules, Bioconjug. Chem. 23, 1934-1944 (2012).
Toshihiko Ooi, Daiki Ogata, Ken’ichiro Matsumoto, Gen Nakamura, Jian Yu, Min Yao, Masaya Kitamura, and Seiichi Taguchi, Flavin-binding of azoreductase: Direct evidences for dual-binding property of apo-azoreductase with FMN and FAD, J. Mol. Cat. B74, 204-208 (2012).
Ryutaro Asano, Makoto Nakayama, Hiroko Kawaguchi, Tsuguo Kubota, Takeshi Nakanishi, Mitsuo Umetsu, Hiroki Hayashi, Yu Katayose, Michiaki Unno, Toshio Kudo, and Izumi Kumagai, Construction and humanization of a functional bispecific EGFR × CD16 diabody using a refolding system, FEBS J. 279, 223-233 (2012).

2011

北村昌也，田口精一，PCR増幅，目的別で選べる核酸実験の原理とプロトコール（羊土社），199-212 (2011)．
北村昌也，田口精一，DNAの変異導入法，目的別で選べる核酸実験の原理とプロトコール（羊土社），213-220 (2011)．
Yasuhiro Watanabe, Ryutaro Asano, Kyoko Arai, Ippei Shimomura, Hiromi Ogata, Hiroko Kawaguchi, Hiroki Hayashi, Hideo Ohtsuka, Hiroshi Yoshida, Yu Katayose, Shinichi Egawa, Takeshi Nakanishi, Mitsuo Umetsu, Hiroshi Yasui, Tadao Ishida, Kohzoh Imai, Toshio Kudo, Michiaki Unno, and Izumi Kumagai, In vitro and in vivo antitumor effects of recombinant bispecific antibodies based on humanized anti-EGFR antibody, Oncol. Rep. 26, 949-955 (2011).
Hideki Watanabe, Kengo Kanazaki, Takeshi Nakanishi, Hidenori Shiotsuka, Satoru Hatakeyama, Masaru Kaieda, Takeshi Imamura, Mitsuo Umetsu, and Izumi Kumagai, Biomimetic engineering of modular bispecific antibodies for biomolecule immobilization, Langmuir 27, 9656-9661 (2011).
Kenji Nakano, Masatoshi Kobayashi, Kei-ichiro Nakamura, Takeshi Nakanishi, Ryutaro Asano, Izumi Kumagai, Hideaki Tahara, Michihiko Kuwano, Justus B. Cohen, and Joseph C. Glorioso, Mechanism of HSV infection through soluble adapter-mediated virus bridging to the EGF receptor, Virology 413, 12-18 (2011).
Ryutaro Asano, Keiko Ikoma, Ippei Shimomura, Shintaro Taki, Takeshi Nakanishi, Mitsuo Umetsu, and Izumi Kumagai, Cytotoxic enhancement of a bispecific diabody (Db) by format conversion to tandem single-chain variable fragment (taFv): THE CASE OF THE hEx3 DIABODY, J. Biol. Chem. 286, 1812-1818 (2011).

2010

Ryutaro Asano, Keiko Ikoma, Yukiko Sone, Hiroko Kawaguchi, Shintaro Taki, Hiroki Hayashi, Takeshi Nakanishi, Mitsuo Umetsu, Yu Katayose, Michiaki Unno, Toshio Kudo, and Izumi Kumagai, Highly enhanced cytotoxicity of a dimeric bispecific diabody, the hEx3 tetrabody, J. Biol. Chem. 285, 20844-20849 (2010).
Haik Chosrowjan, Seiji Taniguchi, Noboru Mataga, Takeshi Nakanishi, Yoshihiro Haruyama, Shuta Sato, Masaya Kitamura, and Fumio Tanaka, The effects of disappearance of one charge on ultrafast fluorescence dynamics of FMN binding protein, J. Phys. Chem. B 114, 6175-6182 (2010).
Mitsuo Umetsu, Takeshi Nakanishi, Ryutaro Asano, Takamitsu Hattori, and Izumi Kumagai, Protein-protein interactions and selection: generation of molecule-binding proteins on the basis of tertiary structural information, FEBS J. 277, 2006-2014 (2010).
Izumi Kumagai, Ryutaro Asano, Takeshi Nakanishi, Kentaro Hashikami, Sho Tanaka, Adel Badran, Hideaki Sanada, and Mitsuo Umetsu, Integration of PEGylation and refolding for renaturation of recombinant proteins from insoluble aggregates produced in bacteria-Application to a single-chain Fv fragment, J. Biosci. Bioeng. 109, 447-452 (2010).
Adel Badran, Ryutaro Asano, Makoto Nakayama, Yasuhiro Watanabe, Takeshi Nakanishi, Mitsuo Umetsu, Hiroki Hayashi, Yu Katayose, Michiaki Unno, and Izumi Kumagai, Target cell-restricted apoptosis induction by 528scFv-TRAIL fusion protein specific for human EGFR and expressed in Escherichia coli, Int. J. Oncol. 36, 1229-1234 (2010).
Takamitsu Hattori, Mitsuo Umetsu, Takeshi Nakanishi, Takanari Togashi, Nozomi Yokoo, Hiroya Abe, Satoshi Ohara, Tadafumi Adschiri, and Izumi Kumagai, High affinity anti-inorganic material antibody generation by integrating graft and evolution technologies: potential of antibodies as biointerface molecules, J. Biol. Chem. 285, 7784-7793 (2010).
Akiko Yokota, Kouhei Tsumoto, Mitsunori Shiroishi, Takeshi Nakanishi, Hidemasa Kondo, and Izumi Kumagai, Contribution of asparagine residues to the stabilization of a proteinaceous antigen-antibody complex, HyHEL-10-hen egg white lysozyme, J. Biol. Chem. 285, 7686-7696 (2010).
Nozomi Yokoo, Takanari Togashi, Mitsuo Umetsu, Kouhei Tsumoto, Takamitsu Hattori, Takeshi Nakanishi, Satoshi Ohara, Seiichi Takami, Takashi Naka, Hiroya Abe, Izumi Kumagai, and Tadafumi Adschiri, Direct and selective immobilization of proteins by means of an inorganic material-binding peptide: Discussion on functionalization in the elongation to material-binding peptide, J. Phys. Chem. B 114, 480-486 (2010).
Ryutaro Asano, Keiko Ikoma, Hiroko Kawaguchi, Yuna Ishiyama, Takeshi Nakanishi, Mitsuo Umetsu, Hiroki Hayashi, Yu Katayose, Michiaki Unno, Toshio Kudo, and Izumi Kumagai, Application of the Fc fusion format to generate tag-free bispecific diabodies, FEBS J. 277, 477-487 (2010).